Why the abundance of RuBisCO in photosynthetic cells may come from its inefficiency

Rubisco, the crucial enzyme powering photosynthesis

Photosynthesis is the crucial process by which plants absorb sunlight, take up water, and breathe in carbon dioxide, prompting chemical reactions that result in the byproducts sugar and oxygen. A key enzyme, rubisco, is responsible for it all, but it is inefficient. This video explores the key protein and reveals fascinating facts about oxygen and photosynthesis.

How MIT researchers have used genetic mutations to improve photosynthesis efficiency

Recognizing that RuBisCO—a protein that binds carbon dioxide to other compounds to produce the building blocks of organic molecules—often mistakenly binds oxygen, scientists performed directed evolution experiments to modify the geometry of a bacterial version of the protein to cause it to preferentially interact with carbon dioxide over oxygen.

The research pipelines being explored by the RIPE Project to improve photosynthesis

These include engineering plants with faster stomata, which would allow photosynthesis to resume more quickly amid fluctuations in available light due to clouds or shading from other plants, and resolving bottlenecks in the regeneration of RuBP, the molecule to which carbon dioxide is fixed during photosynthesis to initiate the Calvin cycle. (Some readers may experience a paywall.)

Understanding the role of photosynthesis as a sink within the carbon cycle

Photosynthetic organisms are net carbon absorbers, pulling carbon dioxide from the atmosphere and storing it in organic compounds and within soil. Approximately one-third of humanity's carbon emissions are stored by these organisms.

An explanation of the Calvin cycle, including why it needs to run six times to create glucose

Each run of this second phase of photosynthesis begins with a five-carbon molecule called RuBP, which gains an additional carbon atom when carbon dioxide (CO₂) is attached. Across six cycles, the six six-carbon molecules—36 carbon atoms in total—are processed into 12 three-carbon sugars, which can be used to generate glucose, and six RuBP molecules, enough for each cycle to restart.

The Amazon rainforest is considered the 'lungs of the Earth,' but it contributes less than a quarter of the oxygen of phytoplankton—microscopic plants, algae, and bacteria capable of photosynthesis.

Instead of consuming water and carbon dioxide to produce oxygen and sugar, anoxygenic photosynthesis involves alternative reactants and products.

An overview of oxygenic photosynthesis, which releases oxygen from breaking down water

Within the membranes of chloroplasts, electrons in pigments are energized by light and transferred across a series of molecules until they create the energetic NADPH molecule. This transfer also powers a hydrogen pump, which creates an ion imbalance that, when relieved, produces energetic ATP molecules. Outside the membranes, both NADPH and ATP are used to synthesize G3Ps, which are funneled into other metabolic pathways to produce sugars.

The history of Earth's breathable atmosphere, made possible by photosynthetic bacteria

Between 3.2 billion and 2.8 billion years ago, threadlike microorganisms called cyanobacteria began consuming available water vapor, carbon dioxide, and sunlight to produce oxygen. However, molecules released from Earth's interior by volcanoes consumed this oxygen via chemical reactions, preventing it from accumulating until volcanism had sufficiently decreased about 2.4 billion years ago.

Artificial photosynthesis turns CO₂ into sustainable fuel

Carbon conversion focuses on a central perplexing question: if carbon dioxide is a leading driver of climate change, and fossil fuels are made of carbon-based molecules, can we devise a way to convert CO₂ into useful alternatives efficiently? This video breaks down a potential pathway using artificial photosynthesis, which uses sunlight to convert CO₂ into fuel.

How artificial photosynthesis can help make plastics and fuels using sunlight

These systems link a molecular light absorber to a catalyst that can synthesize fuels—such as ethanol—and ethylene, a precursor to plastics, in a carbon-neutral manner. Because these byproducts have greater energy concentrations than the carbohydrates produced by photosynthetic organisms, research is ongoing to engineer the necessary catalysts.

How greenhouse gases and climate change impact the efficiency of photosynthesis

A review in the Journal of Experimental Botany found that higher temperatures reduce the efficiency of enzymes involved in photosynthesis and impair a plant's ability to control water loss and regulate carbon dioxide uptake. While increased carbon dioxide levels are understood to accelerate plant growth by boosting photosynthesis, researchers suggest these effects more than offset the potential gain.

Explore the efficiency of photosynthesis under various conditions

In this simulation, users can track how the cellular concentrations of ATP, NADPH, glucose, and oxygen vary over time in response to changes in temperature, acidity, carbon dioxide concentration, and the properties of incoming light. Through multiple trials, users can identify the optimal conditions for photosynthetic reactions.

Carbon sinks absorb more greenhouse gases than they produce

The largest on Earth are oceans, which capture 25% of all carbon emissions and 90% of excess atmospheric thermal radiation while generating half of the world's oxygen. Wildfires and deforestation have reduced forests' net sink capacity, though fungi are believed to capture and store over a third of global carbon emissions.

Early Earth lifeforms likely made energy via mineral reactions, not sunlight

Earth's initial atmosphere was filled with carbon dioxide, methane, and water, with most oxygen locked away in the rocky crust. Over time, bacteria evolved to slowly release that oxygen into the atmosphere by breaking down rock.

Watch a time-lapse from space of photosynthesis across Earth

Using NASA satellite imagery, carbon absorption can be tracked and serves as a proxy for photosynthetic activity on land and in the oceans. Over the course of a year, seasonal changes can be observed as plants become dormant in the fall and active again in the spring.

Single-cell organisms have circadian clocks that direct daily biochemical routines

Cyanobacteria, a type of light-sensitive bacteria, use this clock to signal when to alternate between making organic compounds through photosynthesis and when to convert atmospheric nitrogen into usable ammonia. Upregulating photosynthetic machinery before dawn helps ensure efficient photosynthesis.

How scientists are improving on photosynthesis

Researchers increased yield in soy plants by making them better at photosynthesis, the process that powers life. The findings hold promise for feeding a warming world.

Advances in agriculture are being powered by CRISPR to boost carbon capture

Researchers are using CRISPR to test thousands of plant gene edits, aiming to speed up breeding and maximize carbon retention in soil. By engineering drought tolerance, they also aim to increase crop yields and expand the environments where crops can grow.

Photoautotrophs, which include plants, algae, bacteria, and Euglena, are organisms at the base of the food web that convert light energy from the sun into chemical energy stored in sugars.

Evidence suggests the first cells on Earth were bacteria

About 4 billion years ago, these single-celled organisms harvested carbon dioxide and sunlight to produce their own food and oxygen, setting the stage for the evolution of oxygen-breathing life forms. Archaeabacteria, which thrive on inorganic molecules in extreme habitats on Earth today, were also among the earliest organisms.

Algae can capture carbon dioxide at rates up to 50 times faster than land-based plants and can be grown in tanks that use one-tenth the land footprint of terrestrial plants.

Soil is the largest terrestrial carbon sink on the planet, storing almost twice as much carbon as the atmosphere and all living plants and animals combined.

The three categories of photosynthesis, which each help plants survive in different environments

In the vast majority of plants, C3 photosynthesis produces three-carbon intermediate molecules in the Calvin cycle within the cells of leaves. Plants in hot, dry climates use C4 photosynthesis across two types of cells to produce four-carbon molecules before the Calvin cycle. Plants in drought conditions use CAM to store carbon dioxide as an acid at night, minimizing water loss.

Astronauts on the International Space Station have tested an algae-powered bioreactor that converts carbon dioxide, water, and light into oxygen and edible, protein-rich biomass.

Oxygen and ozone molecules absorb up to 99.9% of UV radiation reaching Earth

Although they make up no more than 10 out of every million molecules in the ozone layer, ozone provides outsized protection by absorbing the most energetic wavelengths of ultraviolet radiation. This absorption increases thermal energy in the stratosphere, causing temperatures there to rise with altitude.

Imagining if humans could perform photosynthesis and how it would change our anatomy

Since chloroplasts reflect green light, becoming photosynthetic would give our skin a green appearance. Because photosynthesis has efficiencies of only 5% to 9%, and humans burn 2,000 Calories daily compared to about 200 burned by a tree, becoming photosynthetic would also require us to adopt a less active, sun-filled lifestyle.

The Google Doodle honoring Jan Ingenhousz, who discovered oxygenic photosynthesis

Born in 1730, the Dutch scientist expanded on existing knowledge that plants produce and absorb gases in his 1779 paper, which detailed that light was necessary for the biochemical processes that produce oxygen in plant leaves. He also identified that all parts of plants produce carbon dioxide at night.

Watch underwater photosynthesis make water look like champagne

In shallow waters where sunlight can reach, photosynthetic reactions absorb water and dissolved carbon dioxide, producing bubbles of oxygen at the surface of plants. As these bubbles rise to the water's surface, a fizzy appearance is created.

Virtually all plants are biofluorescent because their photosynthesis is powered by chlorophyll, a pigment that glows red as a way to get rid of excess energy when exposed to ultraviolet light.

Despite its potential, CRISPR still fails 15% of the time

Biochemists from UIC found that persistent Cas9 binding to double-strand breaks impacts DNA repair efficiency and the effectiveness of the gene editing tool. RNA polymerase collisions also dislodge Cas9, which affects the efficiency of the tool.

The food industry's hard-to-solve pollution

One-quarter of the world's greenhouse gas emissions result from food and agriculture. That whopping number includes production and post-farm processes like distribution. See a visualization of the data along with a breakdown explaining how each process contributes to emissions, and why it’s so difficult to improve the agriculture industry.

Metabolism, a collection of chemical reactions at the cellular level, processes food and converts it into energy to power the body.

Nitrous oxide is the third most significant greenhouse gas made by human activities

Emissions of this molecule increased 40% from 1980 to 2020, with atmospheric concentrations reaching levels higher than at any other time in the last 800,000 years, based on ice core records. Its warming potential is 273 times that of carbon dioxide over 100 years and remains in the atmosphere for more than a century, on average.

The chlorophyll in plants captures energy in the form of red and blue light

Light-dependent reactions during photosynthesis convert sunlight into chemical energy, stored within the bonds of molecules such as ATP and NADPH. Rather than being absorbed for these reactions, most green light is reflected, causing most plants to appear green.

Ozone protects against the sun's UV radiation and enhances the greenhouse effect

In the stratosphere, trioxygen molecules can absorb harmful ultraviolet radiation from the sun, preventing it from reaching Earth's surface, where it can harm organisms and raise surface temperatures. In the lower atmosphere, ozone acts as a greenhouse gas, trapping thermal radiation.

Visualize the purpose of stomata in photosynthesis

These openings on the underside of leaves can be opened and closed by specialized guard cells to regulate the movement of carbon dioxide into and oxygen out of cells, thus impacting the rate of photosynthesis. At night, when the light-dependent reactions in photosynthesis are not possible, the stomata close to prevent the plant from losing water through transpiration.

Carbon dioxide is the most produced greenhouse gas from human activities

In 2022, about 79.7% of US greenhouse gas emissions were carbon dioxide, produced through chemical reactions in processes such as cement production and the burning of fossil fuels, solid waste, trees, and other biological materials. Methane (11.1%), nitrous oxide (6.1%), fluorinated gases (3.1%), and trace amounts of solid and liquid aerosol were also created.

Exposed concrete led to oxygen depletion within Biosphere 2

Instead of being absorbed by plants through photosynthesis to produce oxygen, significant amounts of carbon dioxide were taken up by the calcium hydroxide in the concrete, trapping the oxygen in calcium carbonate and water. This concrete was sealed before the start of the second B2 mission.

Explore the water footprint of producing various proteins, fruits, and vegetables

This interactive gallery highlights the various types of water behind the production of biofuels, foods, and materials such as cotton. Diets high in bovine meat, whose production requires 15,415 liters of water per kilogram (1,847 gallons per pound), are among the most water-intensive, whereas every kilogram of vegetables uses 322 liters of water (39 gal/lb) to produce.

Lining up every microbe on Earth would stretch to about 105,000 light-years

There are about 10²⁷ such organisms on the planet, many of which are adapted to produce energy through uncommon reactions in unconventional environments, such as within rocks or near volcanoes on the ocean floor. Studies of these organisms have reframed astrobiologists' search for life beyond Earth by broadening the parameters under which life can exist.

The ocean's ability to store carbon long-term is being hindered by marine heatwaves

More than 4,000 robotic floats with biogeochemical data sensors, including oxygen, pH, nitrate, suspended particles, chlorophyll, temperature, and conductivity, have been deployed as part of the Global Ocean Biogeochemical Array. They have identified changes to ecosystem structure affecting the ocean's ability to transport carbon from surface waters into the deep.

While carbon capture and storage systems collect carbon dioxide at the source, such as power plants, direct air capture systems extract it from the atmosphere.

Greenhouse gas removal consists of a series of techniques to capture and store GHGs

These include turning biomass into charcoal for storage in soil before it decays and releases carbon dioxide, crushing CO₂-absorbing rocks—increasing exposed surface area—and spreading them across land to accelerate GHG uptake, and restoring degraded peatlands, which can store twice the amount of carbon as the average forest.

How the pigments that absorb light for photosynthesis contribute to plants' green color

Although light energy is captured to power the reactions that produce the building blocks of starch, cellulose, glucose, and other sugars, chlorophyll a and b are most sensitive to violet, blue, orange, and red light, leaving green light mostly unabsorbed. This green light is instead reflected, giving most plants this color appearance.

Cells modify their internal spacing to improve biochemical reaction efficiencies

Studies involving microscopy and genetic engineering tools in yeast and human cells show ribosomes act as crowding agents—much like packing peanuts—and their concentrations are actively tuned to alter the rate of cellular functions. However, these concentrations and cell crowding levels vary across different tissues and cell types.

Since the Industrial Revolution, surface ocean waters have become 30% more acidic due to the absorption of carbon dioxide.

Scientists have collected critical minerals from ocean water using seaweed, a type of algae that leaves behind a mineral-rich powder called bio-ore after being processed into biofuels.

Excluding water vapor, greenhouse gases make up less than 1% of Earth's atmosphere

Although carbon dioxide, methane, nitrous oxide, ozone, and most fluorinated gases account for only a minority of the planet's greenhouse effect due to their lower concentrations, they are more effective at trapping thermal radiation. While water vapor condenses within two weeks, other greenhouse gases linger in the atmosphere for years on average.

A Death Valley shrub optimizes its cells' organelles to photosynthesize in extreme heat

Examining the cellular structure of the plant under heat, scientists observed the formation of novel, cup-shaped chloroplasts and mitochondrial relocation, which shifted the plant's optimal photosynthesis temperature to 45 degrees Celsius (113 degrees Fahrenheit) as it tripled in mass over 10 days. Engineering these mechanisms in crops may help optimize them for hotter environments.