Rubisco, the crucial enzyme powering photosynthesis

Why the abundance of RuBisCO in photosynthetic cells may come from its inefficiency

Despite pulling about 115 billion tons of carbon dioxide from the atmosphere to build organic molecules each year, the enzyme instead binds oxygen at least one in five times, needlessly costing cells energy. To compensate for this and its processing of no more than 10 molecules per second—versus thousands processed by a typical enzyme—it's become the most abundant enzyme on Earth.

An overview of oxygenic photosynthesis, which releases oxygen from breaking down water

Within the membranes of chloroplasts, electrons in pigments are energized by light and transferred across a series of molecules until they create the energetic NADPH molecule. This transfer also powers a hydrogen pump, which creates an ion imbalance that, when relieved, produces energetic ATP molecules. Outside the membranes, both NADPH and ATP are used to synthesize G3Ps, which are funneled into other metabolic pathways to produce sugars.

The research pipelines being explored by the RIPE Project to improve photosynthesis

These include engineering plants with faster stomata, which would allow photosynthesis to resume more quickly amid fluctuations in available light due to clouds or shading from other plants, and resolving bottlenecks in the regeneration of RuBP, the molecule to which carbon dioxide is fixed during photosynthesis to initiate the Calvin cycle. (Some readers may experience a paywall.)

Instead of consuming water and carbon dioxide to produce oxygen and sugar, anoxygenic photosynthesis involves alternative reactants and products.

An explanation of the Calvin cycle, including why it needs to run six times to create glucose

Each run of this second phase of photosynthesis begins with a five-carbon molecule called RuBP, which gains an additional carbon atom when carbon dioxide (CO₂) is attached. Across six cycles, the six six-carbon molecules—36 carbon atoms in total—are processed into 12 three-carbon sugars, which can be used to generate glucose, and six RuBP molecules, enough for each cycle to restart.

How MIT researchers have used genetic mutations to improve photosynthesis efficiency

Recognizing that RuBisCO—a protein that binds carbon dioxide to other compounds to produce the building blocks of organic molecules—often mistakenly binds oxygen, scientists performed directed evolution experiments to modify the geometry of a bacterial version of the protein to cause it to preferentially interact with carbon dioxide over oxygen.

Understanding the role of photosynthesis as a sink within the carbon cycle

Photosynthetic organisms are net carbon absorbers, pulling carbon dioxide from the atmosphere and storing it in organic compounds and within soil. Approximately one-third of humanity's carbon emissions are stored by these organisms.

The chlorophyll in plants captures energy in the form of red and blue light

Light-dependent reactions during photosynthesis convert sunlight into chemical energy, stored within the bonds of molecules such as ATP and NADPH. Rather than being absorbed for these reactions, most green light is reflected, causing most plants to appear green.

Photoautotrophs, which include plants, algae, bacteria, and Euglena, are organisms at the base of the food web that convert light energy from the sun into chemical energy stored in sugars.

The three categories of photosynthesis, which each help plants survive in different environments

In the vast majority of plants, C3 photosynthesis produces three-carbon intermediate molecules in the Calvin cycle within the cells of leaves. Plants in hot, dry climates use C4 photosynthesis across two types of cells to produce four-carbon molecules before the Calvin cycle. Plants in drought conditions use CAM to store carbon dioxide as an acid at night, minimizing water loss.

How the pigments that absorb light for photosynthesis contribute to plants' green color

Although light energy is captured to power the reactions that produce the building blocks of starch, cellulose, glucose, and other sugars, chlorophyll a and b are most sensitive to violet, blue, orange, and red light, leaving green light mostly unabsorbed. This green light is instead reflected, giving most plants this color appearance.

Artificial photosynthesis turns CO₂ into sustainable fuel

Carbon conversion focuses on a central perplexing question: if carbon dioxide is a leading driver of climate change, and fossil fuels are made of carbon-based molecules, can we devise a way to convert CO₂ into useful alternatives efficiently? This video breaks down a potential pathway using artificial photosynthesis, which uses sunlight to convert CO₂ into fuel.

The Amazon rainforest is considered the 'lungs of the Earth,' but it contributes less than a quarter of the oxygen of phytoplankton—microscopic plants, algae, and bacteria capable of photosynthesis.

Visualize the purpose of stomata in photosynthesis

These openings on the underside of leaves can be opened and closed by specialized guard cells to regulate the movement of carbon dioxide into and oxygen out of cells, thus impacting the rate of photosynthesis. At night, when the light-dependent reactions in photosynthesis are not possible, the stomata close to prevent the plant from losing water through transpiration.

Virtually all plants are biofluorescent because their photosynthesis is powered by chlorophyll, a pigment that glows red as a way to get rid of excess energy when exposed to ultraviolet light.

The history of Earth's breathable atmosphere, made possible by photosynthetic bacteria

Between 3.2 billion and 2.8 billion years ago, threadlike microorganisms called cyanobacteria began consuming available water vapor, carbon dioxide, and sunlight to produce oxygen. However, molecules released from Earth's interior by volcanoes consumed this oxygen via chemical reactions, preventing it from accumulating until volcanism had sufficiently decreased about 2.4 billion years ago.

Watch underwater photosynthesis make water look like champagne

In shallow waters where sunlight can reach, photosynthetic reactions absorb water and dissolved carbon dioxide, producing bubbles of oxygen at the surface of plants. As these bubbles rise to the water's surface, a fizzy appearance is created.

Watch a time-lapse from space of photosynthesis across Earth

Using NASA satellite imagery, carbon absorption can be tracked and serves as a proxy for photosynthetic activity on land and in the oceans. Over the course of a year, seasonal changes can be observed as plants become dormant in the fall and active again in the spring.

How artificial photosynthesis can help make plastics and fuels using sunlight

These systems link a molecular light absorber to a catalyst that can synthesize fuels—such as ethanol—and ethylene, a precursor to plastics, in a carbon-neutral manner. Because these byproducts have greater energy concentrations than the carbohydrates produced by photosynthetic organisms, research is ongoing to engineer the necessary catalysts.

The Google Doodle honoring Jan Ingenhousz, who discovered oxygenic photosynthesis

Born in 1730, the Dutch scientist expanded on existing knowledge that plants produce and absorb gases in his 1779 paper, which detailed that light was necessary for the biochemical processes that produce oxygen in plant leaves. He also identified that all parts of plants produce carbon dioxide at night.

Explore the efficiency of photosynthesis under various conditions

In this simulation, users can track how the cellular concentrations of ATP, NADPH, glucose, and oxygen vary over time in response to changes in temperature, acidity, carbon dioxide concentration, and the properties of incoming light. Through multiple trials, users can identify the optimal conditions for photosynthetic reactions.

How scientists are improving on photosynthesis

Researchers increased yield in soy plants by making them better at photosynthesis, the process that powers life. The findings hold promise for feeding a warming world.

How greenhouse gases and climate change impact the efficiency of photosynthesis

A review in the Journal of Experimental Botany found that higher temperatures reduce the efficiency of enzymes involved in photosynthesis and impair a plant's ability to control water loss and regulate carbon dioxide uptake. While increased carbon dioxide levels are understood to accelerate plant growth by boosting photosynthesis, researchers suggest these effects more than offset the potential gain.

Imagining if humans could perform photosynthesis and how it would change our anatomy

Since chloroplasts reflect green light, becoming photosynthetic would give our skin a green appearance. Because photosynthesis has efficiencies of only 5% to 9%, and humans burn 2,000 Calories daily compared to about 200 burned by a tree, becoming photosynthetic would also require us to adopt a less active, sun-filled lifestyle.

Early Earth lifeforms likely made energy via mineral reactions, not sunlight

Earth's initial atmosphere was filled with carbon dioxide, methane, and water, with most oxygen locked away in the rocky crust. Over time, bacteria evolved to slowly release that oxygen into the atmosphere by breaking down rock.

Advances in agriculture are being powered by CRISPR to boost carbon capture

Researchers are using CRISPR to test thousands of plant gene edits, aiming to speed up breeding and maximize carbon retention in soil. By engineering drought tolerance, they also aim to increase crop yields and expand the environments where crops can grow.

How does your body turn food into energy?

Cells get energy via a process known as cellular respiration, which involves turning glucose into ATP or adenosine triphosphate. Breaking the phosphate bonds of ATP results in usable energy being released into cells, which can then use that energy to power biological processes. This 10-minute video explains how glucose turns into ATP, giving the body the energy it needs to survive.

Astronauts on the International Space Station have tested an algae-powered bioreactor that converts carbon dioxide, water, and light into oxygen and edible, protein-rich biomass.

Metabolism, a collection of chemical reactions at the cellular level, processes food and converts it into energy to power the body.

Satellites can be used to measure photosynthetic activity worldwide by observing the red, biofluorescent light emitted by chlorophyll in plants.

Transpiration drives water movement through a plant’s vascular system

Water evaporates from the leaves, creating tension that pulls more water upward from the roots through the xylem—the equivalent of plant blood vessels. This process is supported by water molecules sticking to each other (cohesion) and xylem walls (adhesion).

Ozone protects against the sun's UV radiation and enhances the greenhouse effect

In the stratosphere, trioxygen molecules can absorb harmful ultraviolet radiation from the sun, preventing it from reaching Earth's surface, where it can harm organisms and raise surface temperatures. In the lower atmosphere, ozone acts as a greenhouse gas, trapping thermal radiation.

View examples of luciferin, the light-emitting molecule behind bioluminescence

Bioluminescent marine organisms rely on various unique luciferins, including Cypridina-type luciferin in seed shrimp and chlorophyll-based dinoflagellate luciferin, to react with oxygen to produce light. These chemicals require unique catalysts to help them bind with oxygen.

Explore the water footprint of producing various proteins, fruits, and vegetables

This interactive gallery highlights the various types of water behind the production of biofuels, foods, and materials such as cotton. Diets high in bovine meat, whose production requires 15,415 liters of water per kilogram (1,847 gallons per pound), are among the most water-intensive, whereas every kilogram of vegetables uses 322 liters of water (39 gal/lb) to produce.

How trees transport water from roots to leaves

Trees move water to their extremities via a deft system starting from the roots to the leaves. See how it works in this short video breaking down the process in a Douglas fir.

How Earth's 'greenhouse effect' works

The so-called "greenhouse effect," where various gases in Earth's atmosphere trap heat from the sun, is a crucial factor in our planet's ability to sustain life. It's also pivotal in discussions over humanity's impact on changes in the Earth's...

Single-cell organisms have circadian clocks that direct daily biochemical routines

Cyanobacteria, a type of light-sensitive bacteria, use this clock to signal when to alternate between making organic compounds through photosynthesis and when to convert atmospheric nitrogen into usable ammonia. Upregulating photosynthetic machinery before dawn helps ensure efficient photosynthesis.

Iron rusts in the presence of water and oxygen because these ingredients enable the metal atoms to release energy and enter a more stable state.

What fuels the sun?

The sun is our biggest source of heat and light, but how does this massive powerhouse continue to produce such amazing energy? Basically, it's a fusion reactor. At its core the sun produces all that solar energy through hydrogen fusing and converting into helium, over and over again. Learn more about the entire amazing and explosive process here.

View a gallery of plants made to shine using ultraviolet-induced visible fluorescence photography, in which organisms re-emit the ultraviolet light they are exposed to in vibrant, glowing colors.

Carbon sinks absorb more greenhouse gases than they produce

The largest on Earth are oceans, which capture 25% of all carbon emissions and 90% of excess atmospheric thermal radiation while generating half of the world's oxygen. Wildfires and deforestation have reduced forests' net sink capacity, though fungi are believed to capture and store over a third of global carbon emissions.

Evidence suggests the first cells on Earth were bacteria

About 4 billion years ago, these single-celled organisms harvested carbon dioxide and sunlight to produce their own food and oxygen, setting the stage for the evolution of oxygen-breathing life forms. Archaeabacteria, which thrive on inorganic molecules in extreme habitats on Earth today, were also among the earliest organisms.

An overview of the sun, from its formation to its role in fueling and protecting life on Earth

With a surface gravity almost 28 times Earth's, the sun's mass exerts enough pressure to raise temperatures in its core above 15 million degrees Celsius (27 million degrees Fahrenheit) and enable nuclear fusion. These reactions produce the energy that sustains Earth's photosynthesis-dependent food chains, while the sun's magnetic field protects the solar system from cosmic ionizing radiation.

Some corals absorb harmful ultraviolet radiation and re-emit it as visible light, thereby protecting symbiotic algae within their tissues and providing them with energy for photosynthesis.

Greenhouse gas removal consists of a series of techniques to capture and store GHGs

These include turning biomass into charcoal for storage in soil before it decays and releases carbon dioxide, crushing CO₂-absorbing rocks—increasing exposed surface area—and spreading them across land to accelerate GHG uptake, and restoring degraded peatlands, which can store twice the amount of carbon as the average forest.

A Death Valley shrub optimizes its cells' organelles to photosynthesize in extreme heat

Examining the cellular structure of the plant under heat, scientists observed the formation of novel, cup-shaped chloroplasts and mitochondrial relocation, which shifted the plant's optimal photosynthesis temperature to 45 degrees Celsius (113 degrees Fahrenheit) as it tripled in mass over 10 days. Engineering these mechanisms in crops may help optimize them for hotter environments.

While humans have been genetically modifying plants for millennia by selectively breeding organisms with certain traits, gene-editing tools like CRISPR have further enhanced plants, conferring resistance to drought, pests, and diseases.

The surprising science behind Pop Rocks

Have you ever wondered why Pop Rocks makes tiny explosions in your mouth? Well, there's actually pressurized carbon dioxide bubbles trapped within the sugary candy. This 19-second video shows you what Pop Rocks look like under a microscope.

Learn how chloroplasts—the photosynthesis factories—may have once been independent cells

Possessing DNA of their own that differs from that of their host cell and surrounded by cellular membranes like those observed in bacteria, chloroplasts are believed to have evolved from a cyanobacterial cell. The endosymbiotic theory explains that these organelles were absorbed into larger cells, leading to the photosynthetic cells seen today.

Algae can capture carbon dioxide at rates up to 50 times faster than land-based plants and can be grown in tanks that use one-tenth the land footprint of terrestrial plants.