How do you sequence a genome?

Genomic sequencing is the process of determining the order of nucleotides that make up an organism's genetic information and can be done in about 24 hours or less for human DNA.

Breaking the genome into random segments accelerated the Human Genome Project

Hierarchical shotgun sequencing is a method for determining an organism's genome by fragmenting DNA before sequencing. By creating fragments with overlapping nucleotide bases, the DNA is reassembled by matching fragment ends.

Watch how DNA is packaged to a 10,000th of its original size in a cell

In humans, the 2-meter (6.56-foot) molecule wraps around spool-like proteins called histones, forming beads—nucleosomes—that fold up into fibers. These fibers—chromatin—form loops through folding that compress into larger fibers, which tightly coil together to form a chromosome.

Glowing chemicals allow scientists to read DNA like a barcode

The process of determining the order of nitrogenous bases in DNA involves first cutting the DNA into fragments, which are duplicated multiple times using bacteria. Fluorescently labeled terminator bases are added to fragments, which are sorted by length and read via laser to reconstruct the sequence.

See the history of DNA sequencing

DNA sequencing evolved from manual methods to automated, high-throughput technologies. The Human Genome Project was possible in the first generation of gene sequencing technology with the "chain termination method," which won developer Fredrick Sanger a Nobel Prize.

Watch how Sanger sequencing kickstarted the first generation of DNA sequencing

Sanger sequencing is a foundational DNA sequencing technology developed in 1977. The Nobel Prize-winning discovery uses fluorescent dyes at the end of DNA chains, which are sorted by length through electrophoresis, to determine the order of nucleotide bases.

An overview of the Human Genome Project

The Human Genome Project was a groundbreaking global scientific effort to sequence the genome of humans and several organisms used in scientific research. The sequenced genome was created by combining the DNA of 20 people from Buffalo, New York.

A fascinating look at how our DNA is built and replicated

This easy-to-understand 13-minute video dives into the wondrous molecule deoxyribonucleic acid – also known as DNA – and explains how it replicates itself in our cells.

Visualization of printing synthetic DNA

Ever wonder how synthetic DNA is made? Discover how GenScript uses innovative technology to synthesize precise, high-quality DNA at a larger scale than ever before.

Fully sequenced human genome will help scientists better understand disease

April marks the 20-year anniversary of the completion of the Human Genome Project, a monumental achievement that provided the first map of the human genetic code and advanced our understanding of human biology, disease, and potential treatments....

Here’s how different genetic disorders get passed on

The human genome is complex, as identified by the Human Genome Project. This article digs in to genetic disorders, the most common ones, and how they are diagnosed.

Think we decoded the genome in 2000? Nope. It was 2022.

Two decades after the draft sequence of the human genome was unveiled to great fanfare, a team of 99 scientists has finally deciphered the entire thing. They have filled in vast gaps and corrected a long list of errors in previous versions, giving us a new view of our DNA.

Watch how proteins are created from DNA

When a DNA sequence—a gene—is activated, a copy of the activated portion is created in the form of RNA, a single-stranded version of DNA, in a process known as transcription. This RNA, called messenger RNA, leaves the nucleus and travels to a ribosome, a structure within a cell, which uses it to build a chain of organic compounds that fold into a protein.

Cells translate genetic information into proteins using RNA messengers

Genes are segments of DNA, each composed of a sequence of nitrogenous bases. Within the nucleus, these sequences are copied into a single strand of ribonucleic acid during transcription. This strand leaves the nucleus and is translated by ribosomes into a chain of amino acids that folds into a functional protein.

What is a cell? A great quick overview.

YourGenome.org furnishes a collection of information on cells and how they work. Every body has trillions of cells, each serving a specific function: making proteins, processing chemicals, and creating energy. Every cell contains a genome, a set of instructions for the cell's function. Brush up on your cells for a better grasp on genetics.

Genes are the basic units of heredity

These segments of DNA—long molecules made of nucleotides that coil into chromosomes—code instructions for making specific proteins, which correspond to different traits. Approximately 98 to 99% of human DNA is non-coding, with functions beyond protein synthesis.

Nineteen years after its conclusion, scientists finish what the HGP started

The Human Genome Project mapped 92% of the human genome sequence upon its completion in 2003, leaving unreachable DNA regions unmapped. PacBio HiFi DNA sequencing allowed its completion 19 years later, with the additional mapping of 200 million DNA letters.

Explaining CRISPR at five levels of technical detail

CRISPR is a new area of biomedical science that enables gene editing and could be the key to eventually curing diseases like autism or cancer. WIRED challenges biologist Neville Sanjana to explain this concept to 5 different people; a 7-year-old, a 14-year-old, a college student, a grad student and a CRISPR expert.

Explore the original goals of the Human Genome Project

The project goals emphasized DNA sequencing technologies and methods, which would lead to future treatments of genetic disorders. Exploring the project data's ethical and social implications was also considered.

See a timeline of landmark discoveries in the field of genetics to gain a better understanding of more than a century of advances.

Watch DNA replicate itself before cell division

In the average adult human, between 50 billion and 70 billion cells die daily, requiring them to replicate their genetic instructions before dividing into new cells. This process relies on various proteins to unwind, read, build, and proofread new strands, with error rates of fewer than one per 1 billion nucleotides.

What is genetics?

What is a genome, and how are traits passed from generation to generation? Learn how pea plants helped launch the study of genetics and how the field of genetics research has evolved over time.

The Human Genome Project was forced to accelerate due to a private competitor

Despite a 15-year target when it began in 1990, the Human Genome Project faced competition from Celera Genomics when it entered the sequencing race in 1998. Celera's aim to complete the human genome in three years pressured the HGP to complete their sequencing several years ahead of schedule.

Synthetic biology transforms cells into custom DNA foundries

At companies like Ginkgo Bioworks, researchers are reprogramming cells to act as workshops that can create custom molecules, such as medicines and fertilizers, by stitching together DNA sequences—often in novel combinations—before inserting them into an organism's genome.

In 2025, researchers in the UK began working on developing artificial DNA

The Synthetic Human Genome Project aims to one day construct human DNA, molecule by molecule, in the hopes of creating disease-resistant cells and synthetic chromosomes for study. However, critics warn it may be misused to create enhanced or modified humans.

Visualize how CRISPR-Cas9—a type of immune system used by single-celled organisms to attack invasive viruses—targets and makes precise changes to a gene's DNA.

DNA carries the unique instructions that shape how each person grows and functions

The human genome contains over six billion DNA letters, organized into 46 chromosomes inherited from both parents. Genes—sections of DNA—serve as templates for making proteins, and variations in these sequences can affect how our bodies work or respond to the environment.

Storing data in DNA requires converting binary code into DNA's four-base system

Researchers have suggested that DNA's ability to form compact, dense structures justifies investigating its use for long-term, safe data archiving. This system could fit all of the world's data in a single shoebox, though processing each bit into a nucleotide base takes 2 minutes, as of 2024.

See a timeline of the Human Genome Project

Despite starting on October 1, 1990, the goal of mapping all the DNA found in a human being was first planned in 1984. The project would reach its goals two years ahead of schedule in 2003.

All publicly available DNA sequences are available at GenBank

The National Institutes of Health's database stores comprehensive, annotated DNA sequences as part of the International Nucleotide Sequence Database Collaboration. It encourages unrestricted data access within the scientific community while allowing submitters to retain intellectual property rights over their data.

The Human Genome Project met its goals, but its outcomes were overpromised.

The $3B project scientists called 'junk'

In 1990, scientists set out to map all of human DNA. A $3B bet many called "junk." But the Human Genome Project's radical transparency and a race with a private company sped it to completion in just 13 years. Today, that once-contested map underpins nearly every breakthrough in genetics and personalized medicine.

The secret lives of cells

Cutting-edge microscopy has revealed previously unseen interactions of biological molecules within their cellular environment, enabling scientists to observe and analyze the molecules in a holistic view. This podcast explains the minute advancements made over the last few decades in observing cells, revealing how scientists have come and what the future holds for the field.

Stacking nucleotide molecules like toy blocks helps build DNA's structure

Each nucleotide is made up of a sugar—deoxyribose—a phosphate group, and one of four bases that contain nitrogen. Like building a two-tone, spiral staircase from the ground up, nucleotides are linked one on top of another so that sugars and phosphates alternate. The twisted strands are held together by hydrogen bonds between base pairs.

Like counting how often a word is repeated in a sentence, forensic scientists can identify people by tallying how often short segments of genetic information repeat in specific parts of a person's DNA—a set of numbers that varies from person to person.

Advance genetic research by playing these scientific video games

Computers produce so much information that researchers can't properly analyze all the data produced, and the overload can also lead computers to make mistakes. Researchers are increasingly turning to citizen scientists—members of the public who are willing to volunteer—to help them make sense of data when computers can't. This article details a few of the ways you can contribute to genetics research by playing video games.

The Human Genome Project spawned numerous spinoff genetics projects

The Human Genome Project led to the development of techniques later applied to projects in microbial and plant biology, epigenomics, environmental and cancer genomes, and more. In the spirit of the HGP, data from these projects has been made freely available to develop new technologies.

How do vaccines work? Watch this brief 101

Watch this under-3-minute video explaining how vaccines use the body's immune system to prepare our bodies against viral and bacterial threats to our health.

Understand the complex, biological journey of pregnancy with this science explainer.

A scale of the universe, from atoms to cosmos

Can you really appreciate just how small protons are, or how massive the universe is? To attempt to grasp it, watch this short video which zooms out from a young woman in California to the very limits of the cosmos using established scales. Then, dive deep into the atoms of her white blood cells for a fully-rounded effect.

Different enzymes can be used in the CRISPR system to modify how DNA is cut

The system uses RNA sequences to guide enzymes like Cas9 to specific spots in DNA, where they cut or modify genes. Unlike Cas9, the Cpf1 enzyme makes staggered cuts, which helps insert new DNA more cleanly.

CRISPR-Cas9 enables precise genome editing using a simple RNA-guided system

Jennifer Doudna's research uncovered how bacteria use the tool as an immune defense by cutting viral DNA. She and Emmanuelle Charpentier received the 2020 Nobel Prize in chemistry for showing how this mechanism could be engineered to target and edit genes in living cells.

Breaking down how and why 5G will change the world

The jump from 3G to 4G networks was pretty huge, and 4G to 5G is many times greater and is almost difficult to comprehend. This nine-minute video dives into how 5G will transform our world.

A quick video on how your immune system works

Explore how your immune system's vast network of cells, tissues, and organs coordinate your body's defenses against bacteria, viruses and toxins with this 5-minute TED talk video.

Sex determination isn't all XX and XY

We're generally taught that chromosomes determine an animal's sex, but it is way more nuanced than that. Watch this 12-minute video to find out how.

Explore the differences between healthy and cancerous cells using a DNA microarray

In this simulation, you will learn how scientists use microarrays to experiment on thousands of genes at the same time. By determining which genes are on or off, users can identify those that contribute to cancer formation.

DNA computing allows for storage and processing of massive amounts of data

Although costly and less efficient for simpler tasks, as of 2025, DNA could hold 455 exabytes of data with near limitless parallel computation potential in small data centers. Every movie ever made would fit inside the volume of a single sugar cube.

Strands of DNA can act as biochemical Velcro and fasten two molecules together because the complementary bases that make up DNA strands bond together consistently.

The history of the gene: an interactive timeline

Humans didn’t need modern technology to figure out that kids tend to look like their parents. But what did humans believe about genetics before we knew about DNA or cells in general? Explore this interactive timeline to see how philosophers and scientists throughout the ages tried to explain how traits are passed from generation to generation.

A student researcher working on the Human Genome Project shares their perspective

In a conversation with Ewan Birney, Director of the European Bio-informatics Institute, he discusses what it was like to be a PhD student working on the Human Genome Project in the months before the draft announcement and its impact on him and the field of genetics.