View examples of luciferin, the light-emitting molecule behind bioluminescence

Bioluminescent organisms produce their own light through chemical reactions involving unstable, energetic molecules, while biofluorescent organisms almost immediately re-emit light shone on them, but at a different wavelength.

Bioluminescence provides organisms with a method of hunting, defense, or mating

Unlike glow sticks, bioluminescent reactions use renewable resources like oxygen and ATP. This ability allows fireflies to mate and deter predators, anglerfish to confuse and attract prey, and deep-sea shrimp to defend themselves by vomiting glowing slush.

Bioluminescent reaction enzymes are often used in biomedical research

Near-infrared luciferase systems allow researchers to image and analyze cellular activity in living animals because they penetrate tissue more deeply than visible light. This provides a means of studying gene expression and protein interactions without harming the animals.

View illustrations of bioluminescent functions in marine organisms

Bioluminescence can startle predators, camouflage, lure prey, confuse rivals, or facilitate mate recognition. One tactic—the burglar alarm—involves an organism releasing light to attract a larger predator to its attacker as a means of defense.

Bioluminescence became common in the ocean to support survival in dark environments

Despite seeming like a rare ability in land animals and unseen in plants, bioluminescence has evolved at least 29 times across marine species, indicating its necessity for survival where hiding spots are rare and highlighting how environments provide evolutionary pressure.

View a sample of the bioluminescent tree of life

This diagram shows 42 different light-producing organisms, from bacteria and fungi to worms, fish, and crustaceans. It illustrates how the ability to produce light has appeared multiple times across the tree of life, rather than stemming from a single ancestor.

View a gallery of bioluminescent organisms, including jellyfish and mushrooms

Some species produce a quick flash of light, while others glow steadily with an eerie glow, often to seek attention or in response to being disturbed. Snails, worms, fungi, and other organisms highlight that this ability evolved independently, rather than from one common ancestor.

Massive blooms of microscopic algae called dinoflagellates produce the neon blue light seen in glowing tides.

Humans have used bioluminescence as a means of military advantage

During World War I, the British Navy sank a German U-boat after spotting the blue light forming at the sides and in the wake of the boat. Jellyfish slime was applied to a Roman walking stick for nighttime guidance, while bioluminescent fungi in Indonesia fulfilled the same purpose.

Bioluminescence may provide a sustainable alternative to electric lighting

French start-up Glowee is experimenting with lights made from bioluminescent microorganisms in public spaces to reduce energy use. While the technology shows promise, challenges remain with brightness, maintenance, and scaling it for widespread use.

Why 75% of ocean animals glow

About 75% of ocean creatures can make their own light. It's called bioluminescence—and it all comes down to a special chemical reaction happening inside their bodies.

The first appearance of bioluminescence was at least 540 million years ago

After analyzing fossils, genetic data, and statistical methods, scientists believe octocorals were the first organisms to evolve light-producing abilities. As a result of independent evolution across at least 94 species, this phenomenon is connected to a diverse range of behaviors.

Biofluorescent animals—those that absorb light, such as ultraviolet rays, and re-emit other types of light, such as neon green—include some flying squirrels, scorpions, sea turtles, and platypuses.

Watch David Attenborough's documentary on bioluminescent creatures, which have been observed using light for hunting, mating, communication, and self-defense, and number in the thousands of species.

Bacteria have been genetically engineered to bioluminesce when wine has spoiled

Developed by researchers at Hebrew University, the method involves the firefly enzyme luciferase, which emits light from within bacteria expressing when they are in the presence of acetic acid vapors produced by wine. The process provides affordable, on-site, real-time monitoring of fermentation quality.

Blacklights can illuminate certain biological materials, including blood, teeth, and fingernails, because they contain fluorophores—molecules that visibly glow immediately after exposure to higher-energy light.

Solutions of bioluminescent bacteria can create FOMO-inducing living art

A 2002 exhibition at Montana State University-Bozeman placed the bacteria onto petri dishes that served as macroscopic pixels to create images. Once the bacteria finished eating all the food in the sealed dishes, the light slowly faded away, ending the exhibit.

View a gallery of biofluorescent animals, which contain cells that emit various colors when exposed to ultraviolet light.

When caught in the mouths of cardinal fish, seed shrimp release glowing, bioluminescent chemicals, which alert predators of the cardinal fish, prompting the shrimp's ejection.

Because it is observed in organisms that are not closely related, biofluorescence is thought to have emerged independently many times in nature—over 100 in fish alone.

View a gallery of plants made to shine using ultraviolet-induced visible fluorescence photography, in which organisms re-emit the ultraviolet light they are exposed to in vibrant, glowing colors.

Through sonoluminescence, concentrated sound waves can produce short, high-intensity flashes of light by rapidly collapsing microscopic bubbles to temperatures up to twice that of the sun's surface.

The 2008 Nobel Prize in chemistry was awarded for discovering and developing uses for green fluorescent protein—a biomolecule found in Aequorea victoria, a jellyfish native to the Pacific Northwest.

Fireflies light up to provide mating signals and warnings to predators

Variations in the illumination patterns and flight paths while emitting light have been observed across firefly species. Some species instead rely on pheromones if they are primarily active during the day or have lost the ability to illuminate.

Instead of consuming water and carbon dioxide to produce oxygen and sugar, anoxygenic photosynthesis involves alternative reactants and products.

Virtually all plants are biofluorescent because their photosynthesis is powered by chlorophyll, a pigment that glows red as a way to get rid of excess energy when exposed to ultraviolet light.

An overview of oxygenic photosynthesis, which releases oxygen from breaking down water

Within the membranes of chloroplasts, electrons in pigments are energized by light and transferred across a series of molecules until they create the energetic NADPH molecule. This transfer also powers a hydrogen pump, which creates an ion imbalance that, when relieved, produces energetic ATP molecules. Outside the membranes, both NADPH and ATP are used to synthesize G3Ps, which are funneled into other metabolic pathways to produce sugars.

View an animation of how certain chemicals fluoresce—that is, emit visible light after having absorbed higher-energy light, such as ultraviolet radiation.

Glow sticks feed energy released in chemical reactions to fluorescent dyes

When bent, the glass tube inside the stick breaks, releasing hydrogen peroxide into the surrounding solution. The ensuing reaction creates unstable molecules that decompose and release energy, which is then absorbed by molecular dyes that emit light when their excited electrons return to lower energy states.

Starting with red, colorful objects will appear to lose their colors the deeper they are submerged because water scatters light, reducing the amount available to reflect off objects and be observed.

Watch underwater photosynthesis make water look like champagne

In shallow waters where sunlight can reach, photosynthetic reactions absorb water and dissolved carbon dioxide, producing bubbles of oxygen at the surface of plants. As these bubbles rise to the water's surface, a fizzy appearance is created.

Single-cell organisms have circadian clocks that direct daily biochemical routines

Cyanobacteria, a type of light-sensitive bacteria, use this clock to signal when to alternate between making organic compounds through photosynthesis and when to convert atmospheric nitrogen into usable ammonia. Upregulating photosynthetic machinery before dawn helps ensure efficient photosynthesis.

A nuclear reactor's characteristic blue light is produced from superluminal particles

When light travels through water, its effective speed is 25% lower than its speed in a vacuum. Just as sound waves bunch up and produce a sonic boom when objects move faster than the speed of sound, particles moving faster than this reduced speed generate a shock wave of light, visible as a blue hue.

Scientists can tag distinct proteins with fluorescent probes that emit different colors, allowing them to visualize multiple biochemical processes simultaneously.

By separating light with tools such as prisms, scientists can reveal the presence and composition of celestial matter by identifying color fingerprints unique to specific elements and molecules.

The chlorophyll in plants captures energy in the form of red and blue light

Light-dependent reactions during photosynthesis convert sunlight into chemical energy, stored within the bonds of molecules such as ATP and NADPH. Rather than being absorbed for these reactions, most green light is reflected, causing most plants to appear green.

Random lasers can be created from biomaterials, such as peanuts and birch leaves

Rather than creating a narrow beam of light via amplification in an optical cavity, the random laser produces a red glow by scattering photons created in the natural pores of a peanut kernel after it has been injected with a solution of light-generating carbon dots derived from birch leaves.

How artificial photosynthesis can help make plastics and fuels using sunlight

These systems link a molecular light absorber to a catalyst that can synthesize fuels—such as ethanol—and ethylene, a precursor to plastics, in a carbon-neutral manner. Because these byproducts have greater energy concentrations than the carbohydrates produced by photosynthetic organisms, research is ongoing to engineer the necessary catalysts.

Some female fireflies mimic the signals of other species to lure in and eat them

The genus Photuris does not produce its own defensive pheromones to ward off predators, so it has developed mimicry to attract males of firefly species that do. Unsuspecting males are consumed after these chemicals—lucibufagins—are extracted.

Light pollution is giving oysters insomnia

Oysters, bivalve mollusks that have light-detecting cells along their skin, can't sleep with all this artificial light. According to a 2022 study, oysters' sleep cycles are disturbed by light dimmer than that provided by the full moon. As with humans, it's possible that these bouts of insomnia could have adverse health effects and contribute to the development of disease over time.

View the fluorescently stained internal structures of a tardigrade

The photograph won the Olympus Global Life Science Light Microscopy Americas regional award in 2019. Using chemicals from biofluorescent organisms, the image illuminates internal structures with multiple hues in an otherwise mostly colorless organism.

Some corals absorb harmful ultraviolet radiation and re-emit it as visible light, thereby protecting symbiotic algae within their tissues and providing them with energy for photosynthesis.

The different energy levels of each element result in unique colors when burned

When heated, electrons absorb energy and move to excited states within an atom. They promptly drop back down and release light energy corresponding to the size of the drop, which is unique to each element.

Proteins with limited light sensitivity prevent humans from seeing all types of light

Specialized photoreceptor cells in the retina called cones each possess photopigments that activate when exposed to three ranges of visible light. Other forms of electromagnetic radiation outside the activation range do not generate signals for processing in the brain, thereby limiting average human vision to approximately 10 million colors.

Blood has evolved many times over hundreds of millions of years, resulting in different blood colors and compositions—and there are even some organisms that don't need blood at all.

Photons emitted via stimulated emission have identical characteristics

When confined to a pair of mirrors, a single photon passing a region of population-inverted atoms can produce a chain reaction of monochromatic light. This chain reaction amplifies the initial photon, from which lasers get their name: light amplification by stimulated emission of radiation.

Scientists can create movies of atomic and molecular phenomena using X-ray free-electron lasers, in which images are produced by matter being struck by high-intensity light created by accelerating electrons.

In an X-ray free-electron laser, electromagnetic fields are used to accelerate and move electrons in wiggling patterns, causing them to emit synchronized, high-energy X-rays. This resulting light beam can strike targets up to 120 times per second to create movies of changing molecular structures and phenomena.

Blood color differs across the animal kingdom, with some animals producing blood that appears green (a group of lizards in New Guinea) or blue (horseshoe crabs).

Rubisco, the crucial enzyme powering photosynthesis

Photosynthesis is the crucial process by which plants absorb sunlight, take up water, and breathe in carbon dioxide, prompting chemical reactions that result in the byproducts sugar and oxygen. A key enzyme, rubisco, is responsible for it all, but it is inefficient. This video explores the key protein and reveals fascinating facts about oxygen and photosynthesis.

The term 'symbiosis' was coined during the study of lichens—composite organisms in which algae or cyanobacteria partner with a fungus, alongside bacteria.