Scientists can tag distinct proteins with fluorescent probes that emit different colors, allowing them to visualize multiple biochemical processes simultaneously.

Bioluminescent reaction enzymes are often used in biomedical research

Near-infrared luciferase systems allow researchers to image and analyze cellular activity in living animals because they penetrate tissue more deeply than visible light. This provides a means of studying gene expression and protein interactions without harming the animals.

The 2008 Nobel Prize in chemistry was awarded for discovering and developing uses for green fluorescent protein—a biomolecule found in Aequorea victoria, a jellyfish native to the Pacific Northwest.

View a gallery of biofluorescent animals, which contain cells that emit various colors when exposed to ultraviolet light.

Blacklights can illuminate certain biological materials, including blood, teeth, and fingernails, because they contain fluorophores—molecules that visibly glow immediately after exposure to higher-energy light.

Glowing chemicals allow scientists to read DNA like a barcode

The process of determining the order of nitrogenous bases in DNA involves first cutting the DNA into fragments, which are duplicated multiple times using bacteria. Fluorescently labeled terminator bases are added to fragments, which are sorted by length and read via laser to reconstruct the sequence.

View an animation of how certain chemicals fluoresce—that is, emit visible light after having absorbed higher-energy light, such as ultraviolet radiation.

Biofluorescent animals—those that absorb light, such as ultraviolet rays, and re-emit other types of light, such as neon green—include some flying squirrels, scorpions, sea turtles, and platypuses.

Virtually all plants are biofluorescent because their photosynthesis is powered by chlorophyll, a pigment that glows red as a way to get rid of excess energy when exposed to ultraviolet light.

Bioluminescent organisms produce their own light through chemical reactions involving unstable, energetic molecules, while biofluorescent organisms almost immediately re-emit light shone on them, but at a different wavelength.

Because it is observed in organisms that are not closely related, biofluorescence is thought to have emerged independently many times in nature—over 100 in fish alone.

View a gallery of plants made to shine using ultraviolet-induced visible fluorescence photography, in which organisms re-emit the ultraviolet light they are exposed to in vibrant, glowing colors.

The secret lives of cells

Cutting-edge microscopy has revealed previously unseen interactions of biological molecules within their cellular environment, enabling scientists to observe and analyze the molecules in a holistic view. This podcast explains the minute advancements made over the last few decades in observing cells, revealing how scientists have come and what the future holds for the field.

By separating light with tools such as prisms, scientists can reveal the presence and composition of celestial matter by identifying color fingerprints unique to specific elements and molecules.

Take a 360-degree look at biochemical activity from within a human cell

In this tour, users can observe the dynamic processes that occur within the basic units of the human body, including the production and transport of proteins and the assembly and disassembly of microtubules—thin, cylindrical structures that provide structural support and a means of moving material within the cell.

View the fluorescently stained internal structures of a tardigrade

The photograph won the Olympus Global Life Science Light Microscopy Americas regional award in 2019. Using chemicals from biofluorescent organisms, the image illuminates internal structures with multiple hues in an otherwise mostly colorless organism.

Explore how cells respond to signals from their environment

In "Dropping Signals," users can drag stimuli, such as hormones and light, into various cellular environments to observe cellular responses. These include changing their shape, enabling the transport of chemicals into or out of the cell via the cell membrane, or dividing to compensate for surrounding damaged cells.

Satellites can be used to measure photosynthetic activity worldwide by observing the red, biofluorescent light emitted by chlorophyll in plants.

Glow sticks feed energy released in chemical reactions to fluorescent dyes

When bent, the glass tube inside the stick breaks, releasing hydrogen peroxide into the surrounding solution. The ensuing reaction creates unstable molecules that decompose and release energy, which is then absorbed by molecular dyes that emit light when their excited electrons return to lower energy states.

Cells modify their internal spacing to improve biochemical reaction efficiencies

Studies involving microscopy and genetic engineering tools in yeast and human cells show ribosomes act as crowding agents—much like packing peanuts—and their concentrations are actively tuned to alter the rate of cellular functions. However, these concentrations and cell crowding levels vary across different tissues and cell types.

How radioactive isotopes are created and used for medical diagnostics

In particle accelerators, protons can be accelerated into other atoms to create radioactive isotopes, which can be attached to specific molecules to create radiotracers that are taken up by specific cells. Radiotracers can emit particles that produce light once they collide with an electron, and this light can be captured by a PET scanner to diagnose specific ailments.

Explore what each organelle does within various types of cells

In "Cell Anatomy Viewer," users can explore interactive illustrations of animal, plant, fungal, and bacterial cells. Clicking on an organelle, such as a lysosome, the nucleolus, or the Golgi complex, provides descriptions of its characteristics and functions.

Solutions of bioluminescent bacteria can create FOMO-inducing living art

A 2002 exhibition at Montana State University-Bozeman placed the bacteria onto petri dishes that served as macroscopic pixels to create images. Once the bacteria finished eating all the food in the sealed dishes, the light slowly faded away, ending the exhibit.

In Raman spectroscopy, molecules use some of the energy from incoming light to shift into a more energetic vibration and scatter the remaining light, which scientists can use to confirm the molecule's presence.

Synchrotron radiation has revealed the fur color of creatures millions of years old

When electrons are accelerated in the loop, they produce intense X-rays that can reveal properties of a sample struck by them, just as X-rays can be used to show bone structure in humans. Studying the reflected light from fossils can uncover the presence of known pigmentation compounds.

Bioluminescence provides organisms with a method of hunting, defense, or mating

Unlike glow sticks, bioluminescent reactions use renewable resources like oxygen and ATP. This ability allows fireflies to mate and deter predators, anglerfish to confuse and attract prey, and deep-sea shrimp to defend themselves by vomiting glowing slush.

View examples of luciferin, the light-emitting molecule behind bioluminescence

Bioluminescent marine organisms rely on various unique luciferins, including Cypridina-type luciferin in seed shrimp and chlorophyll-based dinoflagellate luciferin, to react with oxygen to produce light. These chemicals require unique catalysts to help them bind with oxygen.

Cells are categorized by the presence of contained genetic material and other organelles

While prokaryotes contain one or more cells with free-floating genetic material in a region called the nucleoid, eukaryotes possess a nucleus specialized for DNA storage. Plant cells possess cell walls—a reinforced structure surrounding their membrane—made of cellulose, while fungal cell walls contain chitin. Animal cells have lysosomes housing enzymes that break down biomolecules. (Some readers may experience a paywall.)

Bacteria have been genetically engineered to bioluminesce when wine has spoiled

Developed by researchers at Hebrew University, the method involves the firefly enzyme luciferase, which emits light from within bacteria expressing when they are in the presence of acetic acid vapors produced by wine. The process provides affordable, on-site, real-time monitoring of fermentation quality.

Model organisms like yeast, mice, and flies help scientists decode the human body

Some species are used to quickly study the effects of human diseases and treatments because they are short-lived and have similar systems to those found in humans. Bacteria, like E. coli, have been used as human proxies given their rapid growth and ease of genetic manipulability.

PET scans create images of the body by detecting light emitted from the annihilation of matter and antimatter, with the latter produced by custom chemicals containing radioactive atoms.

Nanotechnology refers to the study of objects measuring 1 to 100 billionths of a meter, which has produced materials with unique properties by manipulating the arrangement of atoms.

Like counting how often a word is repeated in a sentence, forensic scientists can identify people by tallying how often short segments of genetic information repeat in specific parts of a person's DNA—a set of numbers that varies from person to person.

Scientists can create movies of atomic and molecular phenomena using X-ray free-electron lasers, in which images are produced by matter being struck by high-intensity light created by accelerating electrons.

In an X-ray free-electron laser, electromagnetic fields are used to accelerate and move electrons in wiggling patterns, causing them to emit synchronized, high-energy X-rays. This resulting light beam can strike targets up to 120 times per second to create movies of changing molecular structures and phenomena.

Explore the differences between healthy and cancerous cells using a DNA microarray

In this simulation, you will learn how scientists use microarrays to experiment on thousands of genes at the same time. By determining which genes are on or off, users can identify those that contribute to cancer formation.

When processing light, the brain can perceive colors with no natural wavelength

While some animals are sensitive to many forms of light, humans possess receptors that are sensitive to red, green, and blue wavelengths. These receptors send signals to the brain, which are processed and mixed to produce the array of colors we observe, including magenta, pink, and "supercolors" that lack a corresponding natural wavelength in the visible spectrum.

Massive blooms of microscopic algae called dinoflagellates produce the neon blue light seen in glowing tides.

Some organisms are composed of a single cell visible to the naked eye

By incorporating tough, cellulose-rich cell walls, rapid movement of molecules via cytoplasmic streaming, and other biological techniques, some unicellular organisms can bypass the physical limitations that restrict the size of cells. Organisms such as the Stentor coeruleus and Acetabularia can be seen without microscopes.

Some organelles are believed to have once been independent cells

Mitochondria and chloroplasts, which are both responsible for energy production in cells, have DNA distinct from that of their host cells and are surrounded by cellular membranes resembling those of bacteria. Through symbiogenesis, these and other specialized structures were absorbed into larger cells, forming the modern-day biological units.

Proteins with limited light sensitivity prevent humans from seeing all types of light

Specialized photoreceptor cells in the retina called cones each possess photopigments that activate when exposed to three ranges of visible light. Other forms of electromagnetic radiation outside the activation range do not generate signals for processing in the brain, thereby limiting average human vision to approximately 10 million colors.

Starting with red, colorful objects will appear to lose their colors the deeper they are submerged because water scatters light, reducing the amount available to reflect off objects and be observed.

Explore the efficiency of photosynthesis under various conditions

In this simulation, users can track how the cellular concentrations of ATP, NADPH, glucose, and oxygen vary over time in response to changes in temperature, acidity, carbon dioxide concentration, and the properties of incoming light. Through multiple trials, users can identify the optimal conditions for photosynthetic reactions.

View a sample of the bioluminescent tree of life

This diagram shows 42 different light-producing organisms, from bacteria and fungi to worms, fish, and crustaceans. It illustrates how the ability to produce light has appeared multiple times across the tree of life, rather than stemming from a single ancestor.

High-powered red light therapy—delivered directly to nerve fibers via a fiber optic needle—has been used in recent experimental research to suppress pain.

View illustrations of bioluminescent functions in marine organisms

Bioluminescence can startle predators, camouflage, lure prey, confuse rivals, or facilitate mate recognition. One tactic—the burglar alarm—involves an organism releasing light to attract a larger predator to its attacker as a means of defense.

How MIT researchers have used genetic mutations to improve photosynthesis efficiency

Recognizing that RuBisCO—a protein that binds carbon dioxide to other compounds to produce the building blocks of organic molecules—often mistakenly binds oxygen, scientists performed directed evolution experiments to modify the geometry of a bacterial version of the protein to cause it to preferentially interact with carbon dioxide over oxygen.

View a gallery of bioluminescent organisms, including jellyfish and mushrooms

Some species produce a quick flash of light, while others glow steadily with an eerie glow, often to seek attention or in response to being disturbed. Snails, worms, fungi, and other organisms highlight that this ability evolved independently, rather than from one common ancestor.

DNA's four-letter blueprint makes the variety of all living things possible

The proteins responsible for cellular functions and an organism's traits are built outside of a cell's nucleus from RNA—single-stranded molecules created by reading the information in DNA. DNA's double-stranded structure makes it more stable and allows RNA to be made from either of its complementary strands.

The mass of a cell can be measured using hollow, vibrating beams

In 2010, scientists at MIT constructed a U-shaped device that vibrates like a guitar string with an internal fluid channel. As cells and bacteria pass through the device, its rate of vibration shifts, with greater mass creating larger shifts.

Harvard and MIT researchers are developing life-saving tattoos

Tattoos aren’t just a fashion statement. Scientists are trying to find life-saving applications for them. Harvard and MIT researchers are developing tattoo inks that react to body chemicals, alerting diabetics to blood sugar fluctuations or athletes to dehydration. Someday, patients may observe their body chemistry with tattoos that change colors.

Learn about the applications of radioactivity in medical, academic, and industrial settings

Besides their use for radiometric dating by archaeologists and researchers at museums and universities, radioactive materials are used in sensors that help track ocean currents and analyze pollution, soil, and petroleum products. Radioisotope-based sensors also help measure air in ice cream and prevent spillovers when soda bottles are filled in factories.