Bioluminescent reaction enzymes are often used in biomedical research

Bioluminescent organisms produce their own light through chemical reactions involving unstable, energetic molecules, while biofluorescent organisms almost immediately re-emit light shone on them, but at a different wavelength.

View examples of luciferin, the light-emitting molecule behind bioluminescence

Bioluminescent marine organisms rely on various unique luciferins, including Cypridina-type luciferin in seed shrimp and chlorophyll-based dinoflagellate luciferin, to react with oxygen to produce light. These chemicals require unique catalysts to help them bind with oxygen.

Bioluminescence provides organisms with a method of hunting, defense, or mating

Unlike glow sticks, bioluminescent reactions use renewable resources like oxygen and ATP. This ability allows fireflies to mate and deter predators, anglerfish to confuse and attract prey, and deep-sea shrimp to defend themselves by vomiting glowing slush.

Bioluminescence became common in the ocean to support survival in dark environments

Despite seeming like a rare ability in land animals and unseen in plants, bioluminescence has evolved at least 29 times across marine species, indicating its necessity for survival where hiding spots are rare and highlighting how environments provide evolutionary pressure.

View illustrations of bioluminescent functions in marine organisms

Bioluminescence can startle predators, camouflage, lure prey, confuse rivals, or facilitate mate recognition. One tactic—the burglar alarm—involves an organism releasing light to attract a larger predator to its attacker as a means of defense.

Blacklights can illuminate certain biological materials, including blood, teeth, and fingernails, because they contain fluorophores—molecules that visibly glow immediately after exposure to higher-energy light.

View a gallery of biofluorescent animals, which contain cells that emit various colors when exposed to ultraviolet light.

Bacteria have been genetically engineered to bioluminesce when wine has spoiled

Developed by researchers at Hebrew University, the method involves the firefly enzyme luciferase, which emits light from within bacteria expressing when they are in the presence of acetic acid vapors produced by wine. The process provides affordable, on-site, real-time monitoring of fermentation quality.

Solutions of bioluminescent bacteria can create FOMO-inducing living art

A 2002 exhibition at Montana State University-Bozeman placed the bacteria onto petri dishes that served as macroscopic pixels to create images. Once the bacteria finished eating all the food in the sealed dishes, the light slowly faded away, ending the exhibit.

Bioluminescence may provide a sustainable alternative to electric lighting

French start-up Glowee is experimenting with lights made from bioluminescent microorganisms in public spaces to reduce energy use. While the technology shows promise, challenges remain with brightness, maintenance, and scaling it for widespread use.

Scientists can tag distinct proteins with fluorescent probes that emit different colors, allowing them to visualize multiple biochemical processes simultaneously.

Humans have used bioluminescence as a means of military advantage

During World War I, the British Navy sank a German U-boat after spotting the blue light forming at the sides and in the wake of the boat. Jellyfish slime was applied to a Roman walking stick for nighttime guidance, while bioluminescent fungi in Indonesia fulfilled the same purpose.

Biofluorescent animals—those that absorb light, such as ultraviolet rays, and re-emit other types of light, such as neon green—include some flying squirrels, scorpions, sea turtles, and platypuses.

View a gallery of bioluminescent organisms, including jellyfish and mushrooms

Some species produce a quick flash of light, while others glow steadily with an eerie glow, often to seek attention or in response to being disturbed. Snails, worms, fungi, and other organisms highlight that this ability evolved independently, rather than from one common ancestor.

Watch David Attenborough's documentary on bioluminescent creatures, which have been observed using light for hunting, mating, communication, and self-defense, and number in the thousands of species.

View a sample of the bioluminescent tree of life

This diagram shows 42 different light-producing organisms, from bacteria and fungi to worms, fish, and crustaceans. It illustrates how the ability to produce light has appeared multiple times across the tree of life, rather than stemming from a single ancestor.

Why 75% of ocean animals glow

About 75% of ocean creatures can make their own light. It's called bioluminescence—and it all comes down to a special chemical reaction happening inside their bodies.

The 2008 Nobel Prize in chemistry was awarded for discovering and developing uses for green fluorescent protein—a biomolecule found in Aequorea victoria, a jellyfish native to the Pacific Northwest.

The first appearance of bioluminescence was at least 540 million years ago

After analyzing fossils, genetic data, and statistical methods, scientists believe octocorals were the first organisms to evolve light-producing abilities. As a result of independent evolution across at least 94 species, this phenomenon is connected to a diverse range of behaviors.

Because it is observed in organisms that are not closely related, biofluorescence is thought to have emerged independently many times in nature—over 100 in fish alone.

View a gallery of plants made to shine using ultraviolet-induced visible fluorescence photography, in which organisms re-emit the ultraviolet light they are exposed to in vibrant, glowing colors.

High-powered red light therapy—delivered directly to nerve fibers via a fiber optic needle—has been used in recent experimental research to suppress pain.

Massive blooms of microscopic algae called dinoflagellates produce the neon blue light seen in glowing tides.

Scientists create contact lenses that give you infrared vision

Scientists have created lenses that use nanoparticles to convert infrared light into visible light, allowing human eyes to observe previously invisible parts of the electromagnetic spectrum. This technology may help colorblind individuals by converting colors they cannot see into wavelengths they can.

Virtually all plants are biofluorescent because their photosynthesis is powered by chlorophyll, a pigment that glows red as a way to get rid of excess energy when exposed to ultraviolet light.

View an animation of how certain chemicals fluoresce—that is, emit visible light after having absorbed higher-energy light, such as ultraviolet radiation.

Light therapy was widely used as a medical treatment for various maladies in the early 1900s before it was replaced by antibiotics.

Scientific applications of bubbles include logic gates, laser production, and medical imaging

Microbubbles of nonreactive gas can be administered to improve the reflection of sound waves and the resulting imaging during an ultrasound. Bouncing light waves within a bubble can stimulate emission of light from a trapped chemical, just as occurs within a laser, and bubbles can act as valves that control the flow and reaction rates in microfluidic systems.

Scientists can create movies of atomic and molecular phenomena using X-ray free-electron lasers, in which images are produced by matter being struck by high-intensity light created by accelerating electrons.

In an X-ray free-electron laser, electromagnetic fields are used to accelerate and move electrons in wiggling patterns, causing them to emit synchronized, high-energy X-rays. This resulting light beam can strike targets up to 120 times per second to create movies of changing molecular structures and phenomena.

Glowing chemicals allow scientists to read DNA like a barcode

The process of determining the order of nitrogenous bases in DNA involves first cutting the DNA into fragments, which are duplicated multiple times using bacteria. Fluorescently labeled terminator bases are added to fragments, which are sorted by length and read via laser to reconstruct the sequence.

The secret lives of cells

Cutting-edge microscopy has revealed previously unseen interactions of biological molecules within their cellular environment, enabling scientists to observe and analyze the molecules in a holistic view. This podcast explains the minute advancements made over the last few decades in observing cells, revealing how scientists have come and what the future holds for the field.

Synchrotron radiation has revealed the fur color of creatures millions of years old

When electrons are accelerated in the loop, they produce intense X-rays that can reveal properties of a sample struck by them, just as X-rays can be used to show bone structure in humans. Studying the reflected light from fossils can uncover the presence of known pigmentation compounds.

NASA began investigating the healing properties of red light therapy in the 1980s, when researchers studying how LED lights could aid in photosynthesis noticed that their hands seemed to heal faster than normal under the lights.

Infrared light is most often associated with thermal radiation and cooking food

Although extensively used in remote controls for electronics, where LED lights send signals wirelessly, infrared cameras allow for night vision and thermography—imaging of heat patterns in the body to non-invasively reveal blood flow for supplementary diagnostics. Infrared radiation also provides heat in toasters, saunas, and other technologies.

View the fluorescently stained internal structures of a tardigrade

The photograph won the Olympus Global Life Science Light Microscopy Americas regional award in 2019. Using chemicals from biofluorescent organisms, the image illuminates internal structures with multiple hues in an otherwise mostly colorless organism.

Through sonoluminescence, concentrated sound waves can produce short, high-intensity flashes of light by rapidly collapsing microscopic bubbles to temperatures up to twice that of the sun's surface.

Random lasers can be created from biomaterials, such as peanuts and birch leaves

Rather than creating a narrow beam of light via amplification in an optical cavity, the random laser produces a red glow by scattering photons created in the natural pores of a peanut kernel after it has been injected with a solution of light-generating carbon dots derived from birch leaves.

Red light therapy—a treatment harnessing a specific form of energy that penetrates the top layers of skin—is marketed for skincare and wellness, but has its roots in NASA plant growth research.

Satellites can be used to measure photosynthetic activity worldwide by observing the red, biofluorescent light emitted by chlorophyll in plants.

Some North American bats can glow in the dark

After examining 60 museum specimens from six different species, scientists found that all of them emitted light after being exposed to ultraviolet radiation. Researchers are perplexed by this natural phenomenon and are currently unable to deduce the reason behind it.

Glow sticks feed energy released in chemical reactions to fluorescent dyes

When bent, the glass tube inside the stick breaks, releasing hydrogen peroxide into the surrounding solution. The ensuing reaction creates unstable molecules that decompose and release energy, which is then absorbed by molecular dyes that emit light when their excited electrons return to lower energy states.

Research studies can be conducted inside or outside of living samples

While "in vitro" experiments occur outside living organisms for cost-effectiveness, "in vivo" experiments take place inside them, providing real-life insights at a higher cost and with increased ethical concerns. Alternatively, "in silico" methods use computational simulations.

Take a 360-degree look at biochemical activity from within a human cell

In this tour, users can observe the dynamic processes that occur within the basic units of the human body, including the production and transport of proteins and the assembly and disassembly of microtubules—thin, cylindrical structures that provide structural support and a means of moving material within the cell.

Proteins with limited light sensitivity prevent humans from seeing all types of light

Specialized photoreceptor cells in the retina called cones each possess photopigments that activate when exposed to three ranges of visible light. Other forms of electromagnetic radiation outside the activation range do not generate signals for processing in the brain, thereby limiting average human vision to approximately 10 million colors.

A 1974 Science News article proclaimed that within a decade, light therapy would be on par with traditional drugs.

Model organisms like yeast, mice, and flies help scientists decode the human body

Some species are used to quickly study the effects of human diseases and treatments because they are short-lived and have similar systems to those found in humans. Bacteria, like E. coli, have been used as human proxies given their rapid growth and ease of genetic manipulability.

Fireflies light up to provide mating signals and warnings to predators

Variations in the illumination patterns and flight paths while emitting light have been observed across firefly species. Some species instead rely on pheromones if they are primarily active during the day or have lost the ability to illuminate.

Scientists using gene therapy recently managed to regenerate retinal cells in the lab—a breakthrough for mammals that could potentially lead to future treatments for human retinal diseases.

Laser technology powers communication, medical and manufacturing tools

Lasers are used when scanning groceries, printing documents or undergoing cosmetic procedures to enhance efficiency, precision and accuracy. Missile defense, targeting and optical communication systems have made using laser technology habitual in the military.

A nuclear reactor's characteristic blue light is produced from superluminal particles

When light travels through water, its effective speed is 25% lower than its speed in a vacuum. Just as sound waves bunch up and produce a sonic boom when objects move faster than the speed of sound, particles moving faster than this reduced speed generate a shock wave of light, visible as a blue hue.