X-ray diffraction reveals atomic structures by analyzing how crystals scatter X-rays

Watch a crystal undergo X-ray diffraction to determine its internal geometry

In the early days of X-ray crystallography, getting photos of a single sample could take dozens of hours, followed by months of mathematical analysis. Today, automation and computer software can complete such tasks in a few hours.

X-rays earned the very first Nobel Prize in physics for revealing hidden worlds

In 1895, Wilhelm Conrad Röntgen discovered X-rays, which could pass through solid objects and make unseen structures visible. Today’s X-ray tools continue to advance science and medicine, including capturing real-time images of proteins.

Photo 51's distinct 'X' pattern and spacing provided data to validate DNA's model

Rosalind Franklin and Raymond Gosling captured the photo after bombarding DNA fibers with X-rays and observing how the beams were deflected. The photo had a 62-hour exposure time and indicated a helical structure for DNA.

Explore a 3D model of the experimental setup Rosalind Franklin used to take Photo 51

To capture the X-ray diffraction image of DNA from the thymus of a calf, Franklin built a humidity-controlling camera to isolate the B form of DNA. The exposure lasted 60 hours and produced a cross pattern, indicating a helical structure.

Rosalind Franklin’s work led to Nobel Prize-winning discoveries in DNA and virology

Her X-ray diffraction imaging helped uncover the structure of DNA, which earned James Watson, Francis Crick, and Maurice Wilkins a Nobel Prize. The same techniques enabled virus imaging, contributing to Aaron Klug's Nobel Prize in chemistry.

As crystals form, their properties and the shapes they take on, including cubic, tetragonal, and hexagonal patterns, reflect the arrangement of their constituent atoms.

Scientists can create movies of atomic and molecular phenomena using X-ray free-electron lasers, in which images are produced by matter being struck by high-intensity light created by accelerating electrons.

In an X-ray free-electron laser, electromagnetic fields are used to accelerate and move electrons in wiggling patterns, causing them to emit synchronized, high-energy X-rays. This resulting light beam can strike targets up to 120 times per second to create movies of changing molecular structures and phenomena.

X-ray data from Rosalind Franklin was key to discovering DNA’s double-helix structure

Although unpublished, the data reached James Watson and Francis Crick, who worked at another institution. Incorporating her data allowed them to validate their model of DNA, which they published without crediting her.

Computed tomography scans use X-rays to create 3D internal images

Just as shadows can reveal the shape of obstacles blocking visible light, the donut-shaped CT scanner measures how X-rays are blocked, revealing the shape of bones, organs, tissue, and other matter along a cross-section. As it rotates, the scanner combines these cross-sections to build a model of the internal structure.

Time crystals are systems of atoms organized into periodic patterns of motion

Unlike traditional crystals—whose atomic structures cause them to look identical no matter how you look at them in space—time crystals exhibit this behavior through time, where the motion of atoms will bring them to the same positions relative to one another regularly.

Particle accelerators have been used to reveal detailed ant anatomy

Using a synchrotron facility at the Karlsruhe Institute of Technology in Germany, a pair of X-ray beams swept across about 2,200 ant specimens to create over 200 terabytes of projection images from multiple angles. The images were compiled into micrometer-resolution 3D reconstructions of 792 species, which are freely accessible on the Antscan platform.

Watson and Crick revealed DNA's double-helix structure using others' key findings

Their 1953 model, made from cardboard cutouts, explained how DNA stores genetic information through base pairing. Rather than experimenting and collecting their own data, they incorporated patterns and data from other scientists through trial and error.

Rosalind Franklin was the long-overlooked contributor to discovering DNA's structure

Though James Watson, Francis Crick, and Maurice Wilkins are credited with discovering the DNA double helix, it incorporated Rosalind Franklin's unpublished data. Photo 51, her famous DNA image, took more than 100 hours to produce and a year’s worth of analysis to interpret.

Airport security scanners use X-rays to infer baggage contents, with newer machines combining images taken at multiple angles and with various X-ray energies for improved threat detection.

Ernest Rutherford's gold foil and nitrogen experiments revealed nuclear structure

Observing high-angle deflections when alpha particles were fired at gold foil led to an atomic model with a dense, positively charged nucleus. Firing those particles at nitrogen resulted in the emission of hydrogen nuclei—protons—leading to the discovery of the subatomic particle.

Synchrotron radiation has revealed the fur color of creatures millions of years old

When electrons are accelerated in the loop, they produce intense X-rays that can reveal properties of a sample struck by them, just as X-rays can be used to show bone structure in humans. Studying the reflected light from fossils can uncover the presence of known pigmentation compounds.

The double-slit experiment is observational evidence for the wave-particle duality

Light and electrons can behave like particles when observed, but they also exhibit wave-like properties in motion, creating patterns through interference. Even larger objects, such as baseballs, have wavelengths, but they are negligibly small compared to their size.

In Raman spectroscopy, molecules use some of the energy from incoming light to shift into a more energetic vibration and scatter the remaining light, which scientists can use to confirm the molecule's presence.

View 3D renderings of atomic orbitals created with quantum mechanics

Scientists use the Schrödinger equation to determine how a system governed by a wavefunction—an equation that predicts the probability of certain outcomes—evolves with time. Computers can animate atomic structure and behavior by depicting the wavefunction as a fluid that guides a particle, like water moving a speck of dust.

Cathode rays are beams of electrons that emit light when hitting a fluorescent screen

When these beams are emitted into an evacuated tube containing charged plates or a nearby magnet, deflections are observed due to the electron charge. In 1897, J.J. Thomson's calculations of this deflection led to his discovery of the subatomic particle.

View the evolution of atomic models, with four successively overturned between 1900 and 1926

Originally thought to be indivisible spheres unique to each element, the discovery of the electron by J.J. Thomson revealed them to be made of smaller particles. Ernest Rutherford's gold foil experiment uncovered the existence of nuclei, which Niels Bohr suggested were surrounded by electrons in fixed orbits. By applying quantum mechanics, Erwin Schrödinger replaced orbits with probability clouds.

In 1880, Jacques and Pierre Curie discovered that electricity could be produced by striking certain materials—the piezoelectric effect—a phenomenon that is seen in barbecue lighters.

Astronauts have used a small portable device to take the first-ever X-rays in space, opening the door for new opportunities for aerospace medicine.

How scientists determine elemental composition based on the light emitted by cooling atoms

When electrons within an atom drop to a lower energy level closer to the nucleus, they lose energy and produce light with energy equal to the difference between the two energy levels. Because these levels and the corresponding emitted light are unique to each element, the light serves as a unique signature that can be compared to known lab samples for identification.

The history of the periodic table and how it hinted at atoms possessing internal structure

In 1869, more than two decades before the discovery of electrons, Russian chemist Dmitrii Mendeleev organized known elements by atomic weight and chemical properties using index cards. His organization revealed previously undiscovered elements and identified patterns in the reactivity of elements, which would later be linked to each element's outermost electron shell. (Some readers may experience a paywall.)

Spikes of light around stars are artifacts of light bending around the mirror supports

Through diffraction, electromagnetic waves can bend around obstacles, as observed in the silver linings at the edges of clouds, where light should be blocked. Within reflecting telescopes, starlight diffracts around struts—beams holding a secondary mirror in place—and spreads perpendicular to them, creating pairs of spikes.

Pierre Curie's knowledge of electromagnetism helped decipher radioactivity

Alongside his brother, he studied electric fields in crystals and the magnetic properties of substances, which required the construction of delicate electrometers. He would use this apparatus with Marie Curie to measure radioactive emissions and the presence of undiscovered radioactive elements.

Before 1932, when particle accelerators were first used for nuclear research, scientists relied on naturally radioactive substances to advance their understanding of the atomic nucleus.

Scientists have determined the properties of cosmic rays by analyzing their trajectories in bubble chambers—containers filled with a vapor that clings to charged particles.

Particle detection has evolved from cloud and bubble chambers to digital 3D displays

As invisible particles passed through cloud and bubble chambers, they disrupted supersaturated vapor and superheated liquid, respectively, creating trails that served as proxies for cosmic rays. The transition from photographed events to digital sensors enhanced data collection and accuracy.

Cosmic rays can be detected using cloud chambers—containers where alcohol vapor condenses around trails of ions created by these high-speed particles from space.

Watch the motion of an individual photon using a high-speed camera

Although light travels over 3 million football fields per second, cameras that capture trillions of frames per second can observe individual particles of light as they interact with atoms in materials they travel through. Such observations can be used to determine the speed of light in different materials.

The Curies developed a way to measure radioactivity in an old medical dissecting room

Despite lacking proper laboratory conditions, Marie and Pierre built an ionization chamber and a piezoelectric quartz electrometer to measure the amount of charged particles released by radiation, with a sensitivity of ten-trillionth of an ampere. Substances with different emission rates indicated different elements.

A composite of telescope images of the sun, which each reveal unique solar features

While traditional cameras can reveal sunspots—cooler regions on the sun's surface—in visible light, x-ray detectors can highlight explosions of high-energy plasma and magnetic fields known as coronal mass ejections. Hydrogen-alpha filters can reveal clouds and filaments of material suspended in magnetic fields above the sun's surface.

Scientists can map Earth's shape and structure by measuring the ratios of certain particles arriving from different directions in space, as these ratios change with the density of the material particles travel through.

Rather than measuring the rising and falling of the sun or pendulum swings, optical clocks track the passage of time using the extremely consistent vibrations of atoms.

The frequency of visible light is significantly greater than that of microwaves used in cesium atomic clocks. This makes optical clocks about 100 times more accurate, losing at most one second across the entire age of the universe.

How atomic clocks have been used to measure the universe's fundamental constants

Atoms behave according to a set of parameters that scientists have determined give reality its properties. For example, the fine-structure constant, α, quantifies the strength of interactions between charged particles. Since the light emitted by atoms as they move between energy levels is affected by α, analyzing these emissions—the basis of atomic clocks—using different atoms helps calculate α.

View the quantum mechanical depiction of electrons as waves around the atomic nucleus

Unlike Bohr's fixed paths, the quantum model describes electrons as exhibiting behavior resembling standing waves—waves that have fixed points at the end while vibrating, like a plucked guitar string. Rather than determining their exact positions, only the probabilities of electrons being in a specific region can be determined.

The first interferometric gravitational wave detector was invented in 1972

Robert Weiss completed the designs after his students asked about an earlier apparatus that used stretched aluminum cylinders to detect passing gravitational waves. Unfamiliar with general relativity and hoping to keep the course focused on experimental tests rather than mathematics, Reiss later asked his students to consider his device for homework. (Some readers may experience a paywall.)

Diamonds have been made from substances such as peanut butter and carbon dioxide, though they can be created from any carbon-rich material under extreme pressure and temperature.

Variations in the energy of light force the use of multiple telescopes

Just as Earth's atmosphere allows the sun's visible light to pass through while blocking other harmful radiation, telescope mirrors and other optics can only reflect, absorb, or transmit certain types of light. Detectors also utilize semiconducting materials that react to specific energies of light, thereby preventing a one-telescope-fits-all solution.

Just as light bends at different angles as it travels through different materials, seismic waves move in different directions through Earth's interior, revealing the presence of layers with varying properties.

Particle accelerators detect subatomic particles using millions of sensors

Calorimeters measure the energy of particles. Tracking devices can measure momentum and charge from the trajectory of particles, which is determined by recording tiny electrical signals. Time-of-flight detectors provide particle velocities directly and particle mass indirectly, giving physicists a suite of properties to compare with those of known matter.

Particle interactions follow rules related to specific quantum properties

Like a business whose purchased inventory matches sold inventory, physicists identified specific patterns across decades of particle accelerator data, which became new conservation laws for elementary particles. These laws help identify which particle decays are expected or forbidden.

Homology-directed repair is the DNA repair method used by cells

Environmental factors like X-ray exposure may break a strand of DNA, but cells can use a sister strand to patch the damage. Scientists exploit this system by introducing custom DNA templates, which cells use instead to make repairs.

The European Space Agency's Euclid telescope maps dark matter

As light rays travel near massive objects in space, their paths can be distorted on their way to Earth, causing objects to appear displaced, magnified, shrunken, or sheared. By simulating these effects for various mass distributions, astronomers can match the distortion to a simulated mass and compare it with the observed visible matter to infer the presence of dark matter.

The history of chemistry up to World War I, from alchemists seeking gold to probing atomic structure

Those involved in the predecessor of the modern scientific discipline engaged in experimentation to develop recipes that would transform ordinary matter into gold and to create medicines, cosmetics, dyes, and alloys. Over the course of centuries, scientists identified unique elements, organized them based on their properties in the periodic table, and eventually developed the technology to discover the internal structure of atoms.

Radio waves are used in broadcasting, weather radar, telecommunications, and GPS

Its ability to bend around obstacles and reflect off the ionosphere—an electrified layer of Earth's upper atmosphere—allows this type of light to travel long distances without damaging living things due to its low energy. MRI scanners, WiFi, and Bluetooth technologies are also applications of this form of light.

View a gallery of Einstein rings

As light from a distant galaxy spreads in all directions, rays that pass around a massive object, such as another galaxy, may be bent around it. If the massive object is positioned between the distant galaxy and our telescopes, we may observe this deflected light forming a ring around it.

Ultrasound machines emit and receive high-frequency sound waves to construct images of the body's interior, with differences in the penetrating power of different frequencies allowing medical professionals to identify different structures.